CD8B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CD8B polyclonal antibody (Cat # PAB3088) in K-562 cell line lysates (35 ug/lane). CD8B (arrow) was detected using the purified polyclonal antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD8B polyclonal antibody (Cat # PAB3088)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD8B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CD8B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CD8B polyclonal antibody (Cat # PAB3088) in K-562 cell line lysates (35 ug/lane). CD8B (arrow) was detected using the purified polyclonal antibody.Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD8B polyclonal antibody (Cat # PAB3088)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CD8B
Entrez GeneID
926Protein Accession#
NP_004922;P10966Gene Name
CD8B
Gene Alias
CD8B1, LYT3, Leu2, Ly3, MGC119115
Gene Description
CD8b molecule
Omim ID
186730Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq
Other Designations
CD8 antigen, beta polypeptide (p37)|CD8 antigen, beta polypeptide 1 (p37)|CD8b antigen|OTTHUMP00000160761|T lymphocyte surface glycoprotein beta chain|T-cell surface glycoprotein CD8 beta chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Recent duplication of the two human CD8 beta-chain genes.
Nakayama K, Kawachi Y, Tokito S, Minami N, Yamamoto R, Imai T, Gachelin G, Nakauchi H.
Journal of Immunology 1992 Mar; 148(6):1919.
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Differential expression and regulation of the human CD8 alpha and CD8 beta chains.
Terry LA, DiSanto JP, Small TN, Flomenberg N.
Tissue Antigens 1990 Feb; 35(2):82.
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Recent duplication of the two human CD8 beta-chain genes.
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