MYLK3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of A-375 cell lysate with MYLK3 polyclonal antibody (Cat # PAB30836). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma with MYLK3 polyclonal antibody (Cat # PAB30836), followed by peroxidase-conjugated secondary antibody and DAB staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYLK3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 40-69 at N-terminus of human MYLK3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Saturated Ammonium Sulfate precipitation
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of A-375 cell lysate with MYLK3 polyclonal antibody (Cat # PAB30836). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma with MYLK3 polyclonal antibody (Cat # PAB30836), followed by peroxidase-conjugated secondary antibody and DAB staining. -
Gene Info — MYLK3
Entrez GeneID
91807Protein Accession#
Q32MK0Gene Name
MYLK3
Gene Alias
MGC126319, MGC126320, MLCK, MLCK2, caMLCK
Gene Description
myosin light chain kinase 3
Gene Ontology
HyperlinkGene Summary
Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM
Other Designations
MLC kinase|cardiac-MyBP-C associated Ca/CaM kinase
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