HIST1H3B3 (phospho S10) polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of HIST1H3B3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S10 of human HIST1H3B3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:10000)
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human hepatocarcinoma with HIST1H3B3 (phospho S10) polyclonal antibody (Cat # PAB30834), followed by peroxidase-conjugated secondary antibody and AEC staining.Dot Blot (Peptide)
Dot blot analysis of HIST1H3B3 (phospho S10) polyclonal antibody (Cat # PAB30834) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — HIST1H3B
Entrez GeneID
8358Protein Accession#
P68431Gene Name
HIST1H3B
Gene Alias
H3/l, H3FL
Gene Description
histone cluster 1, H3b
Omim ID
602819Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member L|OTTHUMP00000016132|histone 1, H3b
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