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Histone H3 (phospho S10) polyclonal antibody

  • Catalog # : PAB30831
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Histone H3.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S10 of human Histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of CEM cell lysate with Histone H3 (phospho S10) polyclonal antibody (Cat # PAB30831).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma with Histone H3 (phospho S10) polyclonal antibody (Cat # PAB30831), followed by peroxidase-conjugated secondary antibody and AEC staining.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3020
  • Gene Name:
  • H3F3A
  • Gene Alias:
  • H3.3A,H3F3,MGC87782,MGC87783
  • Gene Description:
  • H3 histone, family 3A
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000035618,OTTHUMP00000035619,OTTHUMP00000035621
  • Gene Information
  • Entrez GeneID:
  • 3021
  • Gene Name:
  • H3F3B
  • Gene Alias:
  • H3.3B,H3F3A
  • Gene Description:
  • H3 histone, family 3B (H3.3B)
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq
  • Other Designations:
  • H3 histone, family 3A,H3 histone, family 3B
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