FRAT1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of FRAT1 (arrow) using rabbit FRAT1 polyclonal antibody (Cat # PAB3083). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAT1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 polyclonal antibody (Cat # PAB3083) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FRAT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human FRAT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of FRAT1 (arrow) using rabbit FRAT1 polyclonal antibody (Cat # PAB3083). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAT1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 polyclonal antibody (Cat # PAB3083) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — FRAT1
Entrez GeneID
10023Protein Accession#
NP_005470;Q92837Gene Name
FRAT1
Gene Alias
FLJ97193
Gene Description
frequently rearranged in advanced T-cell lymphomas
Omim ID
602503Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. It may function in tumor progression and in lymphomagenesis. [provided by RefSeq
Other Designations
GSK-3 binding protein FRAT1|OTTHUMP00000020201|OTTHUMP00000059391|proto-oncogene FRAT1
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Interactome
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Pathway
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Disease
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Publication Reference
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FRAT1, a substrate-specific regulator of glycogen synthase kinase-3 activity, is a cellular substrate of protein kinase A.
Hagen T, Cross DA, Culbert AA, West A, Frame S, Morrice N, Reith AD.
The Journal of Biological Chemistry 2006 Nov; 281(46):35021.
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Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of beta-catenin in ovarian tumours.
Wang Y, Hewitt SM, Liu S, Zhou X, Zhu H, Zhou C, Zhang G, Quan L, Bai J, Xu N.
British Journal of Cancer 2006 Mar; 94(5):686.
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Casein kinase I epsilon enhances the binding of Dvl-1 to Frat-1 and is essential for Wnt-3a-induced accumulation of beta-catenin.
Hino S, Michiue T, Asashima M, Kikuchi A.
The Journal of Biological Chemistry 2003 Jan; 278(16):14066.
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FRAT1, a substrate-specific regulator of glycogen synthase kinase-3 activity, is a cellular substrate of protein kinase A.
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