CDH13 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDH13.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDH13.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CDH13 (arrow) using rabbit CDH13 polyclonal antibody (Cat # PAB3081). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH13 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDH13 polyclonal antibody (Cat # PAB3081) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CDH13 polyclonal antibody (Cat # PAB3081)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDH13
Entrez GeneID
1012Protein Accession#
NP_001248;P55290Gene Name
CDH13
Gene Alias
CDHH
Gene Description
cadherin 13, H-cadherin (heart)
Omim ID
601364Gene Ontology
HyperlinkGene Summary
This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites. [provided by RefSeq
Other Designations
H-cadherin|P105|T-cad|T-cadherin|cadherin 13|heart-cadherin|truncated-cadherin
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Interactome
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Disease
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Publication Reference
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Identification of a panel of sensitive and specific DNA methylation markers for lung adenocarcinoma.
Tsou JA, Galler JS, Siegmund KD, Laird PW, Turla S, Cozen W, Hagen JA, Koss MN, Laird-Offringa IA.
Molecular Cancer 2007 Oct; 6:70.
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Tumor-specific downregulation and methylation of the CDH13 (H-cadherin) and CDH1 (E-cadherin) genes correlate with aggressiveness of human pituitary adenomas.
Qian ZR, Sano T, Yoshimoto K, Asa SL, Yamada S, Mizusawa N, Kudo E.
Modern Pathology 2007 Dec; 20(12):1269.
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Identification of a panel of sensitive and specific DNA methylation markers for lung adenocarcinoma.
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