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Last updated: 2016/10/23
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CDH13 polyclonal antibody

  • Catalog # : PAB3081
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDH13.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDH13.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of CDH13 (arrow) using rabbit CDH13 polyclonal antibody (Cat # PAB3081). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH13 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDH13 polyclonal antibody (Cat # PAB3081) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using CDH13 polyclonal antibody (Cat # PAB3081)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1012
  • Protein Accession#:
  • NP_001248;P55290
  • Gene Name:
  • CDH13
  • Gene Alias:
  • CDHH
  • Gene Description:
  • cadherin 13, H-cadherin (heart)
  • Gene Summary:
  • This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites. [provided by RefSeq
  • Other Designations:
  • H-cadherin,P105,T-cad,T-cadherin,cadherin 13,heart-cadherin,truncated-cadherin
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