Western blot analysis of CDH9 (arrow) using rabbit CDH9 polyclonal antibody (Cat # PAB3079). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2) (Origene Technologies).
Flow cytometric analysis of MCF-7 cells using CDH9 polyclonal antibody (Cat # PAB3079)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq