CDH9 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CDH9 (arrow) using rabbit CDH9 polyclonal antibody (Cat # PAB3079). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2) (Origene Technologies).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CDH9 polyclonal antibody (Cat # PAB3079)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDH9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDH9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CDH9 (arrow) using rabbit CDH9 polyclonal antibody (Cat # PAB3079). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2) (Origene Technologies).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CDH9 polyclonal antibody (Cat # PAB3079)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDH9
Entrez GeneID
1007Protein Accession#
NP_057363;Q9ULB4Gene Name
CDH9
Gene Alias
MGC125386
Gene Description
cadherin 9, type 2 (T1-cadherin)
Omim ID
609974Gene Ontology
HyperlinkGene Summary
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq
Other Designations
T1-cadherin|cadherin 9, type 2|cadherin-9
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Interactome
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Disease
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Publication Reference
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Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins.
Shimoyama Y, Tsujimoto G, Kitajima M, Natori M.
The Biochemical Journal 2000 Jul; 349(Pt 1):159.
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Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.
Nollet F, Kools P, van Roy F.
Journal of Molecular Biology 2000 Jun; 299(3):551.
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Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue.
Suzuki S, Sano K, Tanihara H.
Cell Regulation 1991 Apr; 2(4):261.
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Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins.
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