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Last updated: 2017/6/25

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ACAT1 polyclonal antibody

  • Catalog # : PAB30781
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant human ACAT1.
  • Immunogen:
  • Recombinant protein corresponding to human ACAT1.
  • Sequence:
  • NEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMV
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
    Western Blot (1:100-1:250)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431 and Lane 4: human liver lysates with ACAT1 polyclonal antibody (Cat # PAB30781).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with ACAT1 polyclonal antibody (Cat # PAB30781) shows strong cytoplasmic positivity in hepatocytes.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U-2 OS with ACAT1 polyclonal antibody (Cat # PAB30781) (Green) shows positivity in mitochondria.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 38
  • Gene Name:
  • ACAT1
  • Gene Alias:
  • ACAT,MAT,T2,THIL
  • Gene Description:
  • acetyl-Coenzyme A acetyltransferase 1
  • Gene Summary:
  • This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq
  • Other Designations:
  • acetoacetyl Coenzyme A thiolase
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