VIL1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with VIL1 polyclonal antibody (Cat # PAB30769) shows strong luminal membranous and cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of CACO-2 with VIL1 polyclonal antibody (Cat # PAB30769) (Green) shows positivity in plasma membrane.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human VIL1.
Immunogen
Recombinant protein corresponding to human VIL1.
Sequence
NGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with VIL1 polyclonal antibody (Cat # PAB30769) shows strong luminal membranous and cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of CACO-2 with VIL1 polyclonal antibody (Cat # PAB30769) (Green) shows positivity in plasma membrane. -
Gene Info — VIL1
Entrez GeneID
7429Protein Accession#
P09327Gene Name
VIL1
Gene Alias
D2S1471, VIL
Gene Description
villin 1
Omim ID
193040Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq
Other Designations
OTTHUMP00000164145
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