RAD9A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human RAD9A.
Immunogen
Recombinant protein corresponding to human RAD9A.
Sequence
AIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with RAD9A polyclonal antibody (Cat # PAB30763) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with RAD9A polyclonal antibody (Cat # PAB30763) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — RAD9A
Entrez GeneID
5883Protein Accession#
Q99638Gene Name
RAD9A
Gene Alias
RAD9
Gene Description
RAD9 homolog A (S. pombe)
Omim ID
603761Gene Ontology
HyperlinkGene Summary
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq
Other Designations
RAD9 homolog|cell cycle checkpoint control protein
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Interactome
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Disease
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