OCLN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with OCLN polyclonal antibody (Cat # PAB30752).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with OCLN polyclonal antibody (Cat # PAB30752) shows moderate cytoplasmic positivity in cells in tubules.
Immunofluorescence
Immunofluorescent staining of A-431 cells with OCLN polyclonal antibody (Cat # PAB30752) (Green) shows positivity in plasma membrane and cell junctions.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human OCLN.
Immunogen
Recombinant protein corresponding to human OCLN.
Sequence
DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with OCLN polyclonal antibody (Cat # PAB30752).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with OCLN polyclonal antibody (Cat # PAB30752) shows moderate cytoplasmic positivity in cells in tubules.Immunofluorescence
Immunofluorescent staining of A-431 cells with OCLN polyclonal antibody (Cat # PAB30752) (Green) shows positivity in plasma membrane and cell junctions. -
Gene Info — OCLN
Entrez GeneID
100506658Protein Accession#
Q16625Gene Name
OCLN
Gene Alias
BLCPMG, PPP1R115, PTORCH1
Gene Description
occludin
Gene Ontology
HyperlinkGene Summary
regulatory subunit 115|tight junction protein occludin
Other Designations
occludin|phosphatase 1, regulatory subunit 115|tight junction protein occludin
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Interactome
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