Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PIK3R3 polyclonal antibody (Cat # PAB30748).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with PIK3R3 polyclonal antibody (Cat # PAB30748) shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunofluorescent staining of U-2 OS with PIK3R3 polyclonal antibody (Cat # PAB30748) (Green) shows positivity in cytoplasm and intermediate filaments.