AATF polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with AATF polyclonal antibody (Cat # PAB30726) shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG with AATF polyclonal antibody (Cat # PAB30726) (Green) shows positivity in nucleus, nucleoli, centrosome and focal adhesion sites.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human AATF.
Immunogen
Recombinant protein corresponding to human AATF.
Sequence
SVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLTSLVGLQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with AATF polyclonal antibody (Cat # PAB30726) shows strong nuclear and cytoplasmic positivity in neuronal cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with AATF polyclonal antibody (Cat # PAB30726) (Green) shows positivity in nucleus, nucleoli, centrosome and focal adhesion sites. -
Gene Info — AATF
Entrez GeneID
26574Protein Accession#
Q9NY61Gene Name
AATF
Gene Alias
CHE-1, CHE1, DED
Gene Description
apoptosis antagonizing transcription factor
Omim ID
608463Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq
Other Designations
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Interactome
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Disease
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