HNRNPD polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with HNRNPD polyclonal antibody (Cat # PAB30723).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with HNRNPD polyclonal antibody (Cat # PAB30723) shows strong nuclear positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG with HNRNPD polyclonal antibody (Cat # PAB30723) (Green) shows positivity in nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human HNRNPD.
Immunogen
Recombinant protein corresponding to human HNRNPD.
Sequence
RGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGDQQSGYGKVS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with HNRNPD polyclonal antibody (Cat # PAB30723).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with HNRNPD polyclonal antibody (Cat # PAB30723) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with HNRNPD polyclonal antibody (Cat # PAB30723) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — HNRNPD
Entrez GeneID
3184Protein Accession#
Q14103Gene Name
HNRNPD
Gene Alias
AUF1, AUF1A, HNRPD, P37, hnRNPD0
Gene Description
heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Omim ID
601324Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq
Other Designations
ARE-binding protein AUFI, type A|AU-rich element RNA-binding protein 1|heterogeneous nuclear ribonucleoprotein D|heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein 1, 37kD)
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Interactome
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