Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: human plasma (IgG/HSA depleted), Lane 4: human liver and Lane 5: human tonsil lysates with USP13 polyclonal antibody (Cat # PAB30714).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with USP13 polyclonal antibody (Cat # PAB30714) shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunofluorescent staining of U-2 OS with USP13 polyclonal antibody (Cat # PAB30714) (Green) shows positivity in cytoplasm and nucleus but excluded from the nucleoli.