Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of human cerebral cortex tissue lysate with ARHGAP1 polyclonal antibody (Cat # PAB30697).
Western Blot (Tissue lysate)
Western Blot analysis of mouse cerebral cortex tissue lysate with ARHGAP1 polyclonal antibody (Cat # PAB30697).
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with ARHGAP1 polyclonal antibody (Cat # PAB30697).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hippocampus (A, B), human stomach (C) with ARHGAP1 polyclonal antibody (Cat # PAB30697). A: Human hippocampus shows positivity in neuropil. B: Human hippocampus shows weak immunoreactivity in neuropil. C: Human stomach shows strong cytoplasmic positivity in glandular cells.
Immunofluorescent staining of U-251 MG (A), mouse dentate gyrus (B), mouse cingulate cortex (C), mouse thalamus (D), mouse motor trigeminal nucleus (E) and mouse brain (F) with ARHGAP1 polyclonal antibody (Cat # PAB30697) (Green). A: U-251 MG shows positivity in vesicles. B: Mouse dentate gyrus shows immunoreactivity in polymorph layer. C: Mouse cingulate cortex shows cytoplasmic immunoreactivity in neurons. D: Mouse thalamus shows positivity in neurons of the anterodorsal thalamic nucleus. E: Mouse motor trigeminal nucleus shows positivity in neuronal cells bodies and processes. F: Mouse brain shows neuronal staining in lateral paragigantocellular nucleus.