TRIM33 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against partial recombinant human TRIM33.
Immunogen
Recombinant protein corresponding to human TRIM33.
Sequence
MQPHLQRQHSNPGHAGPFPVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSR
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with TRIM33 polyclonal antibody (Cat # PAB30690).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hippocampus with TRIM33 polyclonal antibody (Cat # PAB30690) shows strong nuclear positivity in neuronal cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with TRIM33 polyclonal antibody (Cat # PAB30690) (Green) shows positivity in vesicles and nucleus but excluded from the nucleoli. -
Gene Info — TRIM33
Entrez GeneID
51592Protein Accession#
Q9UPN9Gene Name
TRIM33
Gene Alias
FLJ32925, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA
Gene Description
tripartite motif-containing 33
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000013662|OTTHUMP00000013663|ret-fused gene 7|transcriptional intermediary factor 1 gamma|tripartite motif-containing 33 protein
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