RARB polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with RARB polyclonal antibody (Cat # PAB30680).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with RARB polyclonal antibody (Cat # PAB30680) shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of U-2 OS with RARB polyclonal antibody (Cat # PAB30680) (Green) shows positivity in nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human RARB.
Immunogen
Recombinant protein corresponding to human RARB.
Sequence
VLSVSPGQILDFYTASPSSCMLQEKALKACFSGLTQTEWQHRHTAQSIETQSTSSEELVPSPPSPLPPPRVYKPCFVCQDKSSGYHYGV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with RARB polyclonal antibody (Cat # PAB30680).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with RARB polyclonal antibody (Cat # PAB30680) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with RARB polyclonal antibody (Cat # PAB30680) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — RARB
Entrez GeneID
5915Protein Accession#
P10826Gene Name
RARB
Gene Alias
HAP, NR1B2, RRB2
Gene Description
retinoic acid receptor, beta
Omim ID
180220Gene Ontology
HyperlinkGene Summary
This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined. [provided by RefSeq
Other Designations
HBV-activated protein|RAR-epsilon|hepatitis B virus activated protein|retinoic acid receptor beta 2|retinoic acid receptor beta 4|retinoic acid receptor beta 5|retinoic acid receptor, beta polypeptide
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Interactome
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Pathway
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Disease
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