NOVA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human NOVA1.
Immunogen
Recombinant protein corresponding to human NOVA1.
Sequence
NLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with NOVA1 polyclonal antibody (Cat # PAB30679).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with NOVA1 polyclonal antibody (Cat # PAB30679) shows moderate nuclear positivity in neuronal cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with NOVA1 polyclonal antibody (Cat # PAB30679) (Green) shows positivity in nucleoli. -
Gene Info — NOVA1
Entrez GeneID
4857Protein Accession#
P51513Gene Name
NOVA1
Gene Alias
Nova-1
Gene Description
neuro-oncological ventral antigen 1
Omim ID
602157Gene Ontology
HyperlinkGene Summary
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
paraneoplastic Ri antigen|ventral neuron-specific protein 1
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Interactome
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Disease
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