Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with ACSS2 polyclonal antibody (Cat # PAB30675).
Western Blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: human plasma (IgG/HSA depleted), Lane 4: human liver and Lane 5: human tonsil lysates with ACSS2 polyclonal antibody (Cat # PAB30675).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with ACSS2 polyclonal antibody (Cat # PAB30675) shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunofluorescent staining of A-431 cells with ACSS2 polyclonal antibody (Cat # PAB30675) (Green) shows positivity in cytoplasm, vesicles and nucleus but excluded from the nucleoli.
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq
OTTHUMP00000030712,OTTHUMP00000030713,OTTHUMP00000030714,OTTHUMP00000030715,OTTHUMP00000030716,acetate thiokinase,acetate-CoA ligase,acetyl-CoA synthetase,acetyl-Coenzyme A synthetase 2 (ADP forming),acyl-activating enzyme,cytoplasmic acetyl-coenzyme A sy