MARS polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MARS.
Immunogen
Recombinant protein corresponding to human MARS.
Sequence
FVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFD
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with MARS polyclonal antibody (Cat # PAB30671).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex (A, B), human small intestine (C) with MARS polyclonal antibody (Cat # PAB30671). A: Human cerebral cortex shows cytoplasmic positivity in subsets of neurons. B: Human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies. C: Human small intestine shows moderate cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-2 OS (A), mouse cerebellum (B), mouse olfactory bulb (C), mouse visual cortex (D), mouse cerebral peduncle (E) and mouse pons (F) with MARS polyclonal antibody (Cat # PAB30671) (Green). A: U-2 OS shows positivity in cytoplasm. B: Mouse cerebellum shows cytoplasmic immunoreactivity in Purkinje cells. C: Mouse olfactory bulb shows cytoplasmic positivity in mitral and external plexiform layer neurons. D: Mouse visual cortex shows cytoplasmic and axonal staining in neurons. E: Mouse cerebral peduncle shows cytoplasmic positivity in red nucleus neurons. F: Mouse pons shows neuronal positivity in motor trigeminal nucleus. -
Gene Info — MARS
Entrez GeneID
4141Protein Accession#
P56192Gene Name
MARS
Gene Alias
FLJ35667, METRS, MTRNS
Gene Description
methionyl-tRNA synthetase
Omim ID
156560Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [provided by RefSeq
Other Designations
methionine tRNA ligase 1, cytoplasmic|methionine-tRNA synthetase
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Interactome
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Pathway
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Disease
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