PYGL polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PYGL.
Immunogen
Recombinant protein corresponding to human PYGL.
Sequence
VVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: human plasma (IgG/HSA depleted) and Lane 4: human liver lysates with PYGL polyclonal antibody (Cat # PAB30670).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with PYGL polyclonal antibody (Cat # PAB30670) shows strong cytoplasmic positivity in hepatocytes.Immunofluorescence
Immunofluorescent staining of U-2 OS with PYGL polyclonal antibody (Cat # PAB30670) (Green) shows positivity in plasma membrane and cytoplasm. -
Gene Info — PYGL
Entrez GeneID
5836Protein Accession#
P06737Gene Name
PYGL
Gene Alias
GSD6
Gene Description
phosphorylase, glycogen, liver
Omim ID
232700Gene Ontology
HyperlinkGene Summary
This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, or Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
Hers disease|glycogen phosphorylase, liver|glycogen storage disease type VI|phosphorylase, glycogen; liver (Hers disease, glycogen storage disease type VI)
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Interactome
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Pathway
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Disease
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