MIF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) and Lane 3: PC12 cell lysate (pheochromocytoma of rat adrenal medulla) with MIF polyclonal antibody (Cat # PAB30643).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with MIF polyclonal antibody (Cat # PAB30643) shows moderate cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG with MIF polyclonal antibody (Cat # PAB30643) (Green) shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MIF.
Immunogen
Recombinant protein corresponding to human MIF.
Sequence
FIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNN
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) and Lane 3: PC12 cell lysate (pheochromocytoma of rat adrenal medulla) with MIF polyclonal antibody (Cat # PAB30643).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with MIF polyclonal antibody (Cat # PAB30643) shows moderate cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with MIF polyclonal antibody (Cat # PAB30643) (Green) shows positivity in cytoplasm. -
Gene Info — MIF
Entrez GeneID
4282Protein Accession#
P14174Gene Name
MIF
Gene Alias
GIF, GLIF, MMIF
Gene Description
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
Other Designations
glycosylation-inhibiting factor|phenylpyruvate tautomerase
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Interactome
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Pathway
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Disease
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