RBM3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human RBM3.
Immunogen
Recombinant protein corresponding to human RBM3.
Sequence
DEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNY
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with RBM3 polyclonal antibody (Cat # PAB30635).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with RBM3 polyclonal antibody (Cat # PAB30635) shows moderate nuclear positivity in epidermal cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with RBM3 polyclonal antibody (Cat # PAB30635) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — RBM3
Entrez GeneID
5935Protein Accession#
P98179Gene Name
RBM3
Gene Alias
IS1-RNPL, RNPL
Gene Description
RNA binding motif (RNP1, RRM) protein 3
Omim ID
300027Gene Ontology
HyperlinkGene Summary
This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq
Other Designations
OTTHUMP00000025800|OTTHUMP00000025802|RNA binding motif protein 3
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Interactome
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