SEPT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SEPT3 polyclonal antibody (Cat # PAB30628).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lateral ventricle with SEPT3 polyclonal antibody (Cat # PAB30628) shows strong cytoplasmic and nuclear positivity in neuronal cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG with SEPT3 polyclonal antibody (Cat # PAB30628) (Green) shows positivity in plasma membrane and nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SEPT3.
Immunogen
Recombinant protein corresponding to human SEPT3.
Sequence
LSKVVNIIPVIAKADTMTLEEKSEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRVLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SEPT3 polyclonal antibody (Cat # PAB30628).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lateral ventricle with SEPT3 polyclonal antibody (Cat # PAB30628) shows strong cytoplasmic and nuclear positivity in neuronal cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with SEPT3 polyclonal antibody (Cat # PAB30628) (Green) shows positivity in plasma membrane and nucleus but excluded from the nucleoli. -
Gene Info — SEPT3
Entrez GeneID
55964Protein Accession#
Q9UH03Gene Name
SEPT3
Gene Alias
MGC133218, SEP3, bK250D10.3
Gene Description
septin 3
Omim ID
608314Gene Ontology
HyperlinkGene Summary
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000028742|neuronal-specific septin 3
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Interactome
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Disease
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