Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) and Lane 3: PC12 cell lysate (pheochromocytoma of rat adrenal medulla) with AK7 polyclonal antibody (Cat # PAB30627).
Western Blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: human plasma (IgG/HSA depleted), Lane 4: human liver and Lane 5: human tonsil lysates with AK7 polyclonal antibody (Cat # PAB30627).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bronchus with AK7 polyclonal antibody (Cat # PAB30627) shows distinct positivity in cilia of respiratory epithelial cells.
Immunofluorescent staining of U-251 MG with AK7 polyclonal antibody (Cat # PAB30627) (Green) shows positivity in cytoplasm.