ASB7 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ASB7.
Immunogen
Recombinant protein corresponding to human ASB7.
Sequence
HHHCRRNPELQEELQIQAAVAAGDVHTVRKMLEQGYSPNGRDANGWTLLHFSAARGKERCVRVFLEHGADPTVKDLIGGFTALHYAAMHGRARIARLMLESEYRSDIINAKSNDGWTPLHVAAHYGRDSFVRLLLEFKAEVDPLSDK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with ASB7 polyclonal antibody (Cat # PAB30617).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human urinary bladder with ASB7 polyclonal antibody (Cat # PAB30617) shows moderate cytoplasmic positivity in urothelial cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with ASB7 polyclonal antibody (Cat # PAB30617) (Green) shows positivity in cytoplasm and the Golgi apparatus. -
Gene Info — ASB7
Entrez GeneID
140460Protein Accession#
Q9H672Gene Name
ASB7
Gene Alias
FLJ22551
Gene Description
ankyrin repeat and SOCS box-containing 7
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of ankyrin repeat proteins that, along with four other protein families, contains a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. In this way, SOCS box containing proteins may regulate protein turnover by targeting proteins for polyubiquination and, therefore, for proteasome-mediated degradation. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq
Other Designations
ankyrin repeat and SOCS box-containing protein 7
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