Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of human cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).
Western Blot (Tissue lysate)
Western Blot analysis of mouse cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SNAP25 polyclonal antibody (Cat # PAB30613).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with SNAP25 polyclonal antibody (Cat # PAB30613) shows strong cytoplasmic positivity in neuronal and glial cells.
Immunofluorescent staining of A-431 cells with SNAP25 polyclonal antibody (Cat # PAB30613) (Green) shows positivity in cytoplasm, the Golgi apparatus and actin filaments.
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq
OTTHUMP00000030267,OTTHUMP00000030268,resistance to inhibitors of cholinesterase 4 homolog,synaptosomal-associated protein 25