SNAP25 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of human cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).
Western Blot (Tissue lysate)
Western Blot analysis of mouse cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SNAP25 polyclonal antibody (Cat # PAB30613).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with SNAP25 polyclonal antibody (Cat # PAB30613) shows strong cytoplasmic positivity in neuronal and glial cells.
Immunofluorescence
Immunofluorescent staining of A-431 cells with SNAP25 polyclonal antibody (Cat # PAB30613) (Green) shows positivity in cytoplasm, the Golgi apparatus and actin filaments.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SNAP25.
Immunogen
Recombinant protein corresponding to human SNAP25.
Sequence
SSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of human cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).Western Blot (Tissue lysate)
Western Blot analysis of mouse cerebral cortex tissue lysate with SNAP25 polyclonal antibody (Cat # PAB30613).Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SNAP25 polyclonal antibody (Cat # PAB30613).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with SNAP25 polyclonal antibody (Cat # PAB30613) shows strong cytoplasmic positivity in neuronal and glial cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with SNAP25 polyclonal antibody (Cat # PAB30613) (Green) shows positivity in cytoplasm, the Golgi apparatus and actin filaments. -
Gene Info — SNAP25
Entrez GeneID
6616Protein Accession#
P60880Gene Name
SNAP25
Gene Alias
FLJ23079, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2
Gene Description
synaptosomal-associated protein, 25kDa
Omim ID
600322Gene Ontology
HyperlinkGene Summary
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000030267|OTTHUMP00000030268|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein 25
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Interactome
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Pathway
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Disease
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