MYO1C polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MYO1C.
Immunogen
Recombinant protein corresponding to human MYO1C.
Sequence
GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDFTEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEGSTLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with MYO1C polyclonal antibody (Cat # PAB30610).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human gall bladder with MYO1C polyclonal antibody (Cat # PAB30610) shows strong membranous and cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with MYO1C polyclonal antibody (Cat # PAB30610) (Green) shows positivity in plasma membrane and nucleus but excluded from the nucleoli. -
Gene Info — MYO1C
Entrez GeneID
4641Protein Accession#
O00159Gene Name
MYO1C
Gene Alias
FLJ23903, MMI-beta, MMIb, NMI, myr2
Gene Description
myosin IC
Omim ID
606538Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq
Other Designations
myosin-I beta|nuclear myosin I
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