Immunofluorescence (1 - 4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20 - 1:50) Western Blot (1:100 - 1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with PTRH2 polyclonal antibody (Cat # PAB30595) shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
Immunofluorescent staining of human cell line U-2 OS with PTRH2 polyclonal antibody (Cat # PAB30595) shows positivity in mitochondria. Antibody staining is shown in green.