PARP4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis with PARP4 polyclonal antibody (Cat # PAB30582).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human adrenal gland with PARP4 polyclonal antibody (Cat # PAB30582) shows strong nuclear and cytoplasmic positivity in cortical cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with PARP4 polyclonal antibody (Cat # PAB30582) shows positivity in cytoplasm. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PARP4.
Immunogen
Recombinant protein corresponding to amino acids 247-392 of human PARP4.
Sequence
ALLLEEVMNSSTLSQEVSDLVEMIWAEALGHLEHMLLKPVNRISLNDVSKAEGILLLVKAALKNGETAEQLQKMMTEFYRLIPHKGTMPKEVNLGLLAKKADLCQLIRDMVNVCETNLSKPNPPSLAKYRALRCKIEHVEQNTEEF
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 - 1:200)
Western Blot (1:100 - 1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis with PARP4 polyclonal antibody (Cat # PAB30582).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG spImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human adrenal gland with PARP4 polyclonal antibody (Cat # PAB30582) shows strong nuclear and cytoplasmic positivity in cortical cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with PARP4 polyclonal antibody (Cat # PAB30582) shows positivity in cytoplasm. Antibody staining is shown in green. -
Gene Info — PARP4
Entrez GeneID
143Protein Accession#
Q9UKK3Gene Name
PARP4
Gene Alias
ADPRTL1, PARPL, PH5P, VAULT3, VPARP, VWA5C, p193
Gene Description
poly (ADP-ribose) polymerase family, member 4
Omim ID
607519Gene Ontology
HyperlinkGene Summary
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq
Other Designations
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1|H5 proline-rich|I-alpha-I-related|OTTHUMP00000018130|OTTHUMP00000042288|PARP-related|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase-like 1|vault protein, 193-kDa|von Willebrand f
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Interactome
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Pathway
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Disease
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