Immunofluorescence (1 - 4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 - 1:200) Western Blot (1:100 - 1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis with RNF149 polyclonal antibody (Cat # PAB30580). Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine with RNF149 polyclonal antibody (Cat # PAB30580) shows distinct membranous positivity in glandular cells.
Immunofluorescent staining of human cell line U-2 OS with RNF149 polyclonal antibody (Cat # PAB30580) shows positivity in plasma membrane and vesicles. Antibody staining is shown in green.