KIF11 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human KIF11.
Immunogen
Recombinant protein corresponding to amino acids 373-477 of human KIF11.
Sequence
ALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVK
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 - 1:200)
Western Blot (1:100 - 1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis with KIF11 polyclonal antibody (Cat # PAB30576).
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Western Blot (Cell lysate)
Western Blot analysis of human cell line RT-4 with KIF11 polyclonal antibody (Cat # PAB30576).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with KIF11 polyclonal antibody (Cat # PAB30576) shows strong cytoplasmic positivity in cells of seminiferus ducts.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with KIF11 polyclonal antibody (Cat # PAB30576) shows positivity in cytoplasm. Antibody staining is shown in green. -
Gene Info — KIF11
Entrez GeneID
3832Protein Accession#
P52732Gene Name
KIF11
Gene Alias
EG5, HKSP, KNSL1, TRIP5
Gene Description
kinesin family member 11
Omim ID
148760Gene Ontology
HyperlinkGene Summary
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq
Other Designations
OTTHUMP00000020099|kinesin-like 1|kinesin-like spindle protein|kinesin-related motor protein|thyroid receptor interacting protein 5
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Interactome
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Disease
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