Immunofluorescence (1 - 4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500) Western Blot (1:100 - 1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis with HSPA4 polyclonal antibody (Cat # PAB30574). Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Western Blot analysis with HSPA4 polyclonal antibody (Cat # PAB30574). Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human plasma (IgG/HSA depleted) Lane 4: Human liver tissue Lane 5: Human tonsil tissue
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with HSPA4 polyclonal antibody (Cat # PAB30574) shows strong nuclear positivity in cells of seminiferus ducts.
Immunofluorescent staining of human cell line A-431 with HSPA4 polyclonal antibody (Cat # PAB30574) shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Antibody staining is shown in green.