HSPA4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human HSPA4.
Immunogen
Recombinant protein corresponding to amino acids 699-808 of human HSPA4.
Sequence
FEELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDG
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500)
Western Blot (1:100 - 1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis with HSPA4 polyclonal antibody (Cat # PAB30574).
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Western Blot
Western Blot analysis with HSPA4 polyclonal antibody (Cat # PAB30574).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver tissue
Lane 5: Human tonsil tissueImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with HSPA4 polyclonal antibody (Cat # PAB30574) shows strong nuclear positivity in cells of seminiferus ducts.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with HSPA4 polyclonal antibody (Cat # PAB30574) shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — HSPA4
Entrez GeneID
3308Protein Accession#
P34932Gene Name
HSPA4
Gene Alias
APG-2, HS24/P52, MGC131852, RY, hsp70, hsp70RY
Gene Description
heat shock 70kDa protein 4
Omim ID
601113Gene Ontology
HyperlinkGene Summary
isoform a|heat shock protein
Other Designations
heat shock 70kD protein 4|heat shock 70kDa protein 4, isoform a|heat shock protein, 110 kDa
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Interactome
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Pathway
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Disease
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