Immunofluorescence (1 - 4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500) Western Blot (1:100 - 1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with HSF1 polyclonal antibody (Cat # PAB30566) shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.
Immunofluorescent staining of human cell line U-251 MG with HSF1 polyclonal antibody (Cat # PAB30566) shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Antibody staining is shown in green.
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq