HSF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of human cell line PC-3 with HSF1 polyclonal antibody (Cat # PAB30566).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with HSF1 polyclonal antibody (Cat # PAB30566) shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with HSF1 polyclonal antibody (Cat # PAB30566) shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human HSF1.
Immunogen
Recombinant protein corresponding to amino acids 13-160 of human HSF1.
Sequence
SNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTKLLTDVQLMKGKQECMDSKLLA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500)
Western Blot (1:100 - 1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of human cell line PC-3 with HSF1 polyclonal antibody (Cat # PAB30566).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with HSF1 polyclonal antibody (Cat # PAB30566) shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with HSF1 polyclonal antibody (Cat # PAB30566) shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — HSF1
Entrez GeneID
3297Protein Accession#
Q00613Gene Name
HSF1
Gene Alias
HSTF1
Gene Description
heat shock transcription factor 1
Omim ID
140580Gene Ontology
HyperlinkGene Summary
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq
Other Designations
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