PKM2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with PKM2 polyclonal antibody (Cat # PAB30556)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with PKM2 polyclonal antibody (Cat # PAB30556) shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with PKM2 polyclonal antibody (Cat # PAB30556) shows positivity in cytoplasma. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human PKM2.
Immunogen
Recombinant protein corresponding to human PKM2.
Sequence
TATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with PKM2 polyclonal antibody (Cat # PAB30556)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with PKM2 polyclonal antibody (Cat # PAB30556) shows moderate cytoplasmic and membranous positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with PKM2 polyclonal antibody (Cat # PAB30556) shows positivity in cytoplasma. Antibody staining is shown in green. -
Gene Info — PKM2
Entrez GeneID
5315Protein Accession#
P14618Gene Name
PKM2
Gene Alias
CTHBP, MGC3932, OIP3, PK3, PKM, TCB, THBP1
Gene Description
pyruvate kinase, muscle
Omim ID
179050Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
Other Designations
OPA-interacting protein 3|PK, muscle type|pyruvate kinase M2|thyroid hormone-binding protein, cytosolic
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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