SRPK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human SRPK2.
Immunogen
Recombinant protein corresponding to human SRPK2.
Sequence
QQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with SRPK2 polyclonal antibody (Cat # PAB30538)
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cellsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with SRPK2 polyclonal antibody (Cat # PAB30538) shows strong nuclear positivity in purkinje cells and cells of molecular layer.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with SRPK2 polyclonal antibody (Cat # PAB30538) shows positivity in cytoplasm, nucleus and nucleoli. Antibody staining is shown in green. -
Gene Info — SRPK2
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Interactome
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Disease
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