TRAF6 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with TRAF6 polyclonal antibody (Cat # PAB30530)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with TRAF6 polyclonal antibody (Cat # PAB30530) shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with TRAF6 polyclonal antibody (Cat # PAB30530) shows positivity in nucleoli and mitochondria. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human TRAF6.
Immunogen
Recombinant protein corresponding to human TRAF6.
Sequence
SLLNCENSCGSSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQ
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with TRAF6 polyclonal antibody (Cat # PAB30530)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with TRAF6 polyclonal antibody (Cat # PAB30530) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with TRAF6 polyclonal antibody (Cat # PAB30530) shows positivity in nucleoli and mitochondria. Antibody staining is shown in green. -
Gene Info — TRAF6
Entrez GeneID
7189Protein Accession#
Q9Y4K3Gene Name
TRAF6
Gene Alias
MGC:3310, RNF85
Gene Description
TNF receptor-associated factor 6
Omim ID
602355Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq
Other Designations
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