Western blot analysis of HS2ST1 (arrow) using rabbit HS2ST1 polyclonal antibody (Cat # PAB3053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HS2ST1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS2ST1 polyclonal antibody (Cat # PAB3053) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq