HS2ST1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of HS2ST1 (arrow) using rabbit HS2ST1 polyclonal antibody (Cat # PAB3053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HS2ST1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS2ST1 polyclonal antibody (Cat # PAB3053) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HS2ST1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HS2ST1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HS2ST1 (arrow) using rabbit HS2ST1 polyclonal antibody (Cat # PAB3053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HS2ST1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS2ST1 polyclonal antibody (Cat # PAB3053) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — HS2ST1
Entrez GeneID
9653Protein Accession#
Q7LGA3;CAI19004Gene Name
HS2ST1
Gene Alias
FLJ11317, KIAA0448, MGC131986, dJ604K5.2
Gene Description
heparan sulfate 2-O-sulfotransferase 1
Omim ID
604844Gene Ontology
HyperlinkGene Summary
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000011908
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Interactome
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Pathway
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Disease
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