DGKE polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis using DGKE polyclonal antibody (Cat # PAB30518) shows distinct positivity in spermatids and spermatozoa.
Immunofluorescence
Immunofluorescent staining of A-431 cells using DGKE polyclonal antibody (Cat # PAB30518) shows positivity in cytoplasm and nucleus (green) but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human DGKE.
Immunogen
Recombinant protein corresponding to amino acids 63 - 192 of human DGKE.
Sequence
RDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 - 1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis using DGKE polyclonal antibody (Cat # PAB30518) shows distinct positivity in spermatids and spermatozoa.Immunofluorescence
Immunofluorescent staining of A-431 cells using DGKE polyclonal antibody (Cat # PAB30518) shows positivity in cytoplasm and nucleus (green) but excluded from the nucleoli. -
Gene Info — DGKE
Entrez GeneID
8526Protein Accession#
P52429Gene Name
DGKE
Gene Alias
DAGK6, DGK
Gene Description
diacylglycerol kinase, epsilon 64kDa
Omim ID
601440Gene Ontology
HyperlinkGene Summary
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq
Other Designations
diacylglycerol kinase epsilon|diacylglycerol kinase, epsilon (64kD)
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Interactome
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Pathway
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Disease
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