SIGLEC1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis with SIGLEC1 polyclonal antibody (Cat # PAB30284).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver tissue
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung with SIGLEC1 polyclonal antibody (Cat # PAB30284) shows distinct membranous positivity in macrophages.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SIGLEC1.
Immunogen
Recombinant protein corresponding to amino acids 1425-1510 of human SIGLEC1.
Sequence
CTAQNLLGSISTIGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYH
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500)
Western Blot (1:500 - 1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis with SIGLEC1 polyclonal antibody (Cat # PAB30284).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver tissueImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung with SIGLEC1 polyclonal antibody (Cat # PAB30284) shows distinct membranous positivity in macrophages. -
Gene Info — SIGLEC1
Entrez GeneID
6614Protein Accession#
Q9BZZ2Gene Name
SIGLEC1
Gene Alias
CD169, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, SIGLEC-1, SN, dJ1009E24.1
Gene Description
sialic acid binding Ig-like lectin 1, sialoadhesin
Omim ID
600751Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000030124|sialic acid-binding immunoglobulin-like lectin 1|sialoadhesin
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Interactome
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Pathway
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