HMOX1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human HMOX1.
Immunogen
Recombinant protein corresponding to amino acids of human HMOX1.
Sequence
QDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAF
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot (Cell lysate) analysis with HMOX1 polyclonal antibody (Cat # PAB30262) at 1:250 - 1:500 dilution
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bone marrow with HMOX1 polyclonal antibody (Cat # PAB30262) shows strong positivity in macrophages at 1:500 - 1:1000 dilution. -
Gene Info — HMOX1
Entrez GeneID
3162Protein Accession#
P09601Gene Name
HMOX1
Gene Alias
HO-1, HSP32, bK286B10
Gene Description
heme oxygenase (decycling) 1
Omim ID
141250Gene Ontology
HyperlinkGene Summary
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq
Other Designations
OTTHUMP00000028925|heme oxygenase (decyclizing) 1
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Interactome
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Pathway
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Disease
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