HSPB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human HSPB1.
Immunogen
Recombinant protein corresponding to amino acids of human HSPB1.
Sequence
EEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot (Cell lysate) analysis with HSPB1 polyclonal antibody (Cat # PAB30258) at 1:250 - 1:500 dilution
Lane 1: Human cell line RT-4
Lane 2: Human cell line EFO-21
Lane 3: Human cell line A-431
Lane 4: Human liver tissueImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human smooth muscle with HSPB1 polyclonal antibody (Cat # PAB30258) shows strong cytoplasmic positivity in smooth muscle cells at 1:50 - 1:200 dilution. -
Gene Info — HSPB1
Entrez GeneID
3315Protein Accession#
P04792Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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