ATG4C polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with ATG4C polyclonal antibody (Cat # PAB30239) at 1:100 - 1:250 dilution
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with ATG4C polyclonal antibody (Cat # PAB30239) shows strong cytoplasmic positivity in myocytes at 1:20 - 1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with ATG4C polyclonal antibody (Cat # PAB30239) shows positivity in cytoplasm and nucleus but excluded from the nucleoli, under 1-4 ug/mL concentration. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ATG4C.
Immunogen
Recombinant protein corresponding to amino acids of human ATG4C.
Sequence
EEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with ATG4C polyclonal antibody (Cat # PAB30239) at 1:100 - 1:250 dilution
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with ATG4C polyclonal antibody (Cat # PAB30239) shows strong cytoplasmic positivity in myocytes at 1:20 - 1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with ATG4C polyclonal antibody (Cat # PAB30239) shows positivity in cytoplasm and nucleus but excluded from the nucleoli, under 1-4 ug/mL concentration. Antibody staining is shown in green. -
Gene Info — ATG4C
Entrez GeneID
84938Protein Accession#
Q96DT6Gene Name
ATG4C
Gene Alias
APG4-C, APG4C, AUTL1, AUTL3, FLJ14867
Gene Description
ATG4 autophagy related 4 homolog C (S. cerevisiae)
Omim ID
611339Gene Ontology
HyperlinkGene Summary
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq
Other Designations
APG4 autophagy 4 homolog C|AUT-like 1, cysteine endopeptidase|AUT-like 3 cysteine endopeptidase|OTTHUMP00000010715|autophagin-3|autophagy-related cysteine endopeptidase 3
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Interactome
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Pathway
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