Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50) Immunofluorescence (1-4 ug/mL) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate) analysis with ATG4C polyclonal antibody (Cat # PAB30239) at 1:100 - 1:250 dilution Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with ATG4C polyclonal antibody (Cat # PAB30239) shows strong cytoplasmic positivity in myocytes at 1:20 - 1:50 dilution.
Immunofluorescent staining of human cell line U-251 MG with ATG4C polyclonal antibody (Cat # PAB30239) shows positivity in cytoplasm and nucleus but excluded from the nucleoli, under 1-4 ug/mL concentration. Antibody staining is shown in green.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq