INS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with INS polyclonal antibody (Cat # PAB30238) at 1:100 - 1:250 dilution
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with INS polyclonal antibody (Cat # PAB30238) shows cytoplasmic positivity in islets of Langerhans at 1:2500 - 1:5000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human INS.
Immunogen
Recombinant protein corresponding to amino acids of human INS.
Sequence
SHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYC
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:2500-1:5000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with INS polyclonal antibody (Cat # PAB30238) at 1:100 - 1:250 dilution
Lane 1: Negative control (vector only transfected HEK293T lysate)
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with INS polyclonal antibody (Cat # PAB30238) shows cytoplasmic positivity in islets of Langerhans at 1:2500 - 1:5000 dilution. -
Gene Info — INS
Entrez GeneID
3630Protein Accession#
P01308Gene Name
INS
Gene Alias
ILPR, IRDN
Gene Description
insulin
Gene Ontology
HyperlinkGene Summary
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq
Other Designations
OTTHUMP00000011161|OTTHUMP00000011162|OTTHUMP00000196038|proinsulin
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Interactome
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Pathway
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Disease
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