Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Immunofluorescence (1-4 ug/mL) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate) analysis with GNPTG polyclonal antibody (Cat # PAB30236) at 1:100 - 1:250 dilution Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with GNPTG polyclonal antibody (Cat # PAB30236) shows strong cytoplasmic positivity in glandular cells at 1:50 - 1:200 dilution.
Immunofluorescent staining of human cell line U-2 OS with GNPTG polyclonal antibody (Cat # PAB30236) shows positivity in endoplasmic reticulum under 1-4 ug/mL concentration. Antibody staining is shown in green.
UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-phosphotransferase) catalyzes the initial step in the synthesis of the mannose 6-phosphate determinant required for efficient intracellular targeting of newly synthesized lysosomal hydrolases to the lysosome. GlcNAc-phosphotransferase is an alpha-2/beta-2/gamma-2 hexameric complex, the enzyme product of 2 genes, 1 encoded by a gene on chromosome 16 (GNPTG) and the other by a gene on chromosome 12 (GNPTAB; MIM 607840).[supplied by OMIM