Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ODF2 polyclonal antibody (Cat #PAB30222).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis shows moderate cytoplasmic and nuclear positivity in seminiferous ducts with ODF2 polyclonal antibody (Cat # PAB30222).
The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of this gene; however, not all isoforms and variants have been fully described. [provided by RefSeq
OTTHUMP00000022273,OTTHUMP00000022274,cenexin 1,outer dense fiber of sperm tails, 84-kD,outer dense fibre of sperm tails 2,sperm tail structural protein