Product Browser

Last updated: 2016/12/4

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

POLR2A CTD repeat (phospho S5) polyclonal antibody

  • Catalog # : PAB30192
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human POLR2A CTD repeat (phospho S5).
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S5 of human POLR2A CTD repeat.
  • Sequence:
  • Y-S-P-T-Sp-P-S
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 217
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C or lower.
    Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
    The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of MCF-7 cell lysates with POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192).
    Lane 1: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192)
    Lane 2: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with phospho-peptide
    Lane 3: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with non-phospho-peptide
    Lane 4: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with generic [pS] peptide
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5430
  • Gene Name:
  • POLR2A
  • Gene Alias:
  • MGC75453,POLR2,POLRA,RPB1,RPBh1,RPO2,RPOL2,RpIILS,hRPB220,hsRPB1
  • Gene Description:
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • Gene Summary:
  • This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq
  • Other Designations:
  • DNA directed RNA polymerase II polypeptide A,DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit,polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • RSS
  • YouTube
  • Linkedin
  • Facebook