Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human POLR2A CTD repeat (phospho S2).
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S2 of human POLR2A CTD repeat.
Theoretical MW (kDa):
This antibody detects endogenous level of POLR2A CTD repeat YSPTSPS, only when phosphorylated at serine 2 of YSPTSPS. This antibody has no cross-reactivity with RNA polymerase II, when phosphorylated at serine of YSPTSPS.
Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq
DNA directed RNA polymerase II polypeptide A,DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit,polymerase (RNA) II (DNA directed) polypeptide A (220kD)