Rabbit polyclonal antibody raised against partial synthetic peptide of human LMNA.
A synthetic peptide (conjugated with KLH) corresponding to residues within 400-450 of human LMNA.
Flow Cytometry Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: Hela, Lane 2: A431, Lane 3: HepG2, Lane 4: LOVO, Lane 5: NIH/3T3 cell lysates with LMNA polyclonal antibody (Cat # PAB30180). Predicted Size: Lamin A: 71 KDa and Lamin C: 65 Kda Observed Size: Lamin A: 71 KDa and Lamin C: 65 Kda
Flow cytometric analysis of HepG2 cells with LMNA polyclonal antibody (Cat # PAB30180) (shaded histogram). Open histogram: isotype control.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq